Amplified sequences from chromosome 15, including centromeres, nucleolar organizer regions, and centromeric heterochromatin, in homogeneously staining regions in the human melanoma cell line MeWo

Abstract

Homogeneously staining regions (HSRs) in the human melanoma cell line, MeWo, are located on an X and a der(15) chromosome. These regions are homogeneously stained with quinacrine fluorescence, but stain differentially with conventional Giemsa, G-banding, C-banding, and distamycin A/4',6-diamidino-2-phenylindole. There are five and six blocks of positively staining material on the X and der(15) HSRs, respectively. Hybridization in situ with a cloned repetitive Kpn I family member has confirmed the amplification of this sequence along the HSRs. With silver staining of the nucleolar organizer regions (NORs) there appear to be three very strong and two weaker pairs of NORs along the HSR of the X chromosome and four strong and one weaker pair on that of the der(15) chromosome. There was little NOR staining on the normal acrocentric chromosomes in these cells, suggesting preferential transcription of the NORs in the HSRs. Centromere-dot staining revealed a distribution of multiple centromeres similar to the NORs along the two HSRs. These data suggest that a unit composed of the short arm and centromere of chromosome #15 has been amplified and that the HSR present on the X chromosome probably arose by a translocation from chromosome #15.