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Clinical Trial
. 2015 Mar 17;10(3):e0121087.
doi: 10.1371/journal.pone.0121087. eCollection 2015.

Effect of oral glucose administration on rebound growth hormone release in normal and obese women: the role of adiposity, insulin sensitivity and ghrelin

Lara Pena-Bello  1 , Sonia Pertega-Diaz  2 , Elena Outeiriño-Blanco  3 , Jesus Garcia-Buela  4 , Sulay Tovar  5 , Susana Sangiao-Alvarellos  1 , Carlos Dieguez  5 , Fernando Cordido  6
Affiliations
Clinical Trial

Effect of oral glucose administration on rebound growth hormone release in normal and obese women: the role of adiposity, insulin sensitivity and ghrelin

Lara Pena-Bello et al. PLoS One. .

Abstract

Context: Metabolic substrates and nutritional status play a major role in growth hormone (GH) secretion. Uncovering the mechanisms involved in GH secretion following oral glucose (OG) administration in normal and obese patients is a pending issue.

Objective: The aim of this study was to investigate GH after OG in relation with adiposity, insulin secretion and action, and ghrelin secretion in obese and healthy women, to further elucidate the mechanism of GH secretion after OG and the altered GH secretion in obesity.

Participants and methods: We included 64 healthy and obese women. After an overnight fast, 75 g of OG were administered; GH, glucose, insulin and ghrelin were obtained during 300 minutes. Insulin secretion and action indices and the area under the curve (AUC) were calculated for GH, glucose, insulin and ghrelin. Univariate and multivariate linear regression analyses were employed.

Results: The AUC of GH (μg/L•min) was lower in obese (249.8±41.8) than in healthy women (490.4±74.6), P=0.001. The AUC of total ghrelin (pg/mL•min) was lower in obese (240995.5±11094.2) than in healthy women (340797.5±37757.5), P=0.042. There were significant correlations between GH secretion and the different adiposity, insulin secretion and action, and ghrelin secretion indices. After multivariate analysis only ghrelin AUC remained a significant predictor for fasting and peak GH.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Mean±SE plasma glucose (a; mg/dL), insulin (b; μU/mL) GH (c; μg/L) and total ghrelin levels (d; pg/mL) in normal (grey) and obese women (black) during the prolongued oral glucose tolerance test.
* p<0.05 between normal and obese women at that time point. Insulin was higher in the obese women than in the healthy control women after the 300 min OG (Fig. 1b). GH was lower in the the obese women than in the healthy control women after OG (Fig. 1c). The AUC of GH (μg/L·min) between 0 and 300 min was lower in the obese patients than in the controls; 249.8±41.8 vs 490.4±74.6, P = 0.001, for the obese patients and controls respectively. Peak GH (μg/L) levels were lower in the obese than in the healthy women, 3.3±0.5 vs 6.6±0.8 p<0.001, for the obese and healthy women respectively. Total ghrelin was lower in the obese women than in the healthy women after OG (Fig. 1d). The AUC of total ghrelin (pg/mL·min) between 0 and 300 min was lower in the obese patients than in the controls; 240995.5±11094.2 vs 340797.5±37757.5, P = 0.042, for the obese and healthy women respectively (Fig. 2).
Fig 2
Fig 2. Mean±SE plasma GH AUC0–300 (pg/mL·min) and Ghrelin AUC0–300 (pg/mL·min) in normal (grey) and obese women (black) during the prolongued oral glucose tolerance test.
* p<0.05 between normal and obese women.
Fig 3
Fig 3. Relationship between GH secretion indices (log-transformed) and IGF-I, waist circumference, Matsuda index and Ghrelin AUC0–300 data.
Univariate generalized additive regression (GAM) models. A clear linear association between ghrelin AUC and the different indices of GH secretion (log-transformed) was found (Fig. 3).
See this image and copyright information in PMC

References

    1. Rasmussen MH, Hvidberg A, Juul A, Main KM, Gotfredsen A, Skakkebaek NE, et al. Massive weight loss restores 24-hour growth hormone release profiles and serum insulin-like growth factor-I levels in obese subjects. J Clin Endocrinol Metab. 1995;80(4):1407–15. Epub 1995/04/01. - PubMed
    1. Cordido F, Penalva A, Dieguez C, Casanueva FF. Massive growth hormone (GH) discharge in obese subjects after the combined administration of GH-releasing hormone and GHRP-6: evidence for a marked somatotroph secretory capability in obesity. J Clin Endocrinol Metab. 1993;76(4):819–23. Epub 1993/04/01. - PubMed
    1. Berryman DE, Glad CA, List EO, Johannsson G. The GH/IGF-1 axis in obesity: pathophysiology and therapeutic considerations. Nat Rev Endocrinol. 2013;9(6):346–56. Epub 2013/04/10. 10.1038/nrendo.2013.64 - DOI - PubMed
    1. Huang L, Steyn FJ, Tan HY, Xie TY, Veldhuis JD, Ngo ST, et al. The Decline in Pulsatile GH Secretion throughout Early Adulthood in Mice Is Exacerbated by Dietary-Induced Weight Gain. Endocrinology. 2012;153(9):4380–8. Epub 2012/07/18. 10.1210/en.2012-1178 - DOI - PubMed
    1. Luque RM, Gahete MD, Valentine RJ, Kineman RD. Examination of the direct effects of metabolic factors on somatotrope function in a non-human primate model, Papio anubis. J Mol Endocrinol. 2006;37(1):25–38. Epub 2006/08/12. - PubMed

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