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Comment
. 2015 Apr;83(4):1732-3.
doi: 10.1128/IAI.02892-14.

A truncation in the regulator RocA underlies heightened capsule expression in serotype M3 group A streptococci

N N Lynskey  1 , C E Turner  1 , Li S Heng  1 , S Sriskandan  2
Affiliations
Comment

A truncation in the regulator RocA underlies heightened capsule expression in serotype M3 group A streptococci

N N Lynskey et al. Infect Immun. 2015 Apr.
No abstract available

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Figures

FIG 1
FIG 1
Schematic of serotype M3 RocA truncation. Linear representations of M1 and M3 RocA. The gray bars denote the precise location of the predicted ATPase domain. The black arrow indicates the location of the conserved frameshift mutation at codon 410 of M3 RocA. The black box represents a subsequent sequence alteration and a premature stop codon which results in the truncation of the M3 RocA protein at amino acid 416.
FIG 2
FIG 2
Serotype M3 capsule expression is derepressed by RocA M3 truncation. (A) M3 GAS express more capsule than M1 (n = 5 strains/group); however, capsule levels in M3 GAS were reduced by overexpression of full-length RocA (B) or chromosomal integration of a single copy of full-length RocA (C). All strains were wild type for CovR/S. M3 strains contained serotype-specific mutations in fasC, rivR, and rocA; M1 strains were wild type for these regulators. Data represent means (plus standard deviations) of results from 3 or 4 individual experiments. ND, not detected (below the limit of detection); *, Mann-Whitney P < 0.05.
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References

    1. Cao TN, Liu Z, Cao TH, Pflughoeft KJ, Treviño J, Danger JL, Beres SB, Musser JM, Sumby P. 2014. Natural disruption of two regulatory networks in serotype M3 group A Streptococcus isolates contributes to the virulence factor profile of this hypervirulent serotype. Infect Immun 82:1744–1754. doi:10.1128/IAI.01639-13. - DOI - PMC - PubMed
    1. Lynskey NN, Goulding D, Gierula M, Turner CE, Dougan G, Edwards RJ, Sriskandan S. 2013. RocA truncation underpins hyper-encapsulation, carriage longevity and transmissibility of serotype M18 group A streptococci. PLoS Pathog 9:e1003842. doi:10.1371/journal.ppat.1003842. - DOI - PMC - PubMed

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