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. 1985;65(1):89-91.

Increased sensitivity of the fluorescent treponemal antibody absorption test with biotin/avidin: a comparison with conventional FTA-ABS

  • PMID: 2578717

Increased sensitivity of the fluorescent treponemal antibody absorption test with biotin/avidin: a comparison with conventional FTA-ABS

E Berntsson. Acta Derm Venereol. 1985.

Abstract

The biotin/avidin system was adapted to the FTA technique for detection of IgG, IgM, and IgA antibodies specific to Treponema pallidum. By using biotin/avidin IgG antibodies could be detected in a serum dilution 15 000 times higher, and IgM 3 000 times higher than was possible with conventional FTA technique. IgA antibodies, however, were not detected with any of the methods.

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