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. 2015 Mar 19:5:9262.
doi: 10.1038/srep09262.

The role of activated cytotoxic T cells in etiopathogenesis of periodontal disease: does it harm or does it heal?

Emine Cifcibasi  1 , Meral Ciblak  2 , Bayram Kiran  3 , Selim Badur  2 , Erhan Firatli  1 , Halim Issever  4 , Serdar Cintan  1
Affiliations

The role of activated cytotoxic T cells in etiopathogenesis of periodontal disease: does it harm or does it heal?

Emine Cifcibasi et al. Sci Rep. .

Abstract

The objective of this study was to determine the phenotypic profile of blood mononuclear cells, specifically CD8(+)/CD28(+) cells, in patients with generalized aggressive periodontitis (GAgP) and chronic periodontitis (CP) in peripheral blood and in blood obtained from periodontal defect site which might contribute to tissue damage. 13 GAgP, 11 chronic periodontitis (CP) and 5 healthy controls (H) were included in the study. Plaque index (PI), bleeding on probing (BoP), periodontal probing depth (PPD) and clinical attachment level (CAL) were recorded. Blood from the base of periodontal defect site and peripheral blood from the antecubital vein were obtained. Relative counts of CD45(+), CD3(+), CD4(+), CD8(+)/CD28(+), CD8(+)/CD28(-), CD19(+), CD16(+)/CD56(+)/CD3, CD3(+)/CD16(+)/CD56(+) receptors were determined with two color flow cytometry using monoclonal antibodies. BoP, PPD and CAL were significantly higher in both periodontitis groups than healthy controls (p <0.05). Activated cytotoxic T cells, CD8(+)/CD28(+) cells, were significantly elevated in GAgP and CP groups compared to HC both in blood obtained from defect site and blood obtained from systemic circulation (p <0.05). GAgP and CP patients have an increased levels of activated cytotoxic T cells as a result of inflammation which may cause severe tissue damage that lead to severe and rapid loss of periodontal tissues.

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Figures

Figure 1
Figure 1. Flow cytometric analysis using six color Mab on FACSCanto with FACCSDiva software version 6.1.3. of a representative case.
Figure 2
Figure 2. Flow Cytometric Analysis Using Double Mab CD8/CD28 Kit on FACSCanto with FACSDiva Software Version 6.1.3. of a representative case.
Figure 3
Figure 3. Phenotypic lymphocyte subset analysis of GAgP, CP and HC groups in blood from the defect site (% proportion of lymphocytes positive for the given monoclonal antibodies)
Figure 4
Figure 4. Comparison of % proportion of lymphocytes positive for the various monoclonal antibodies in peripheral blood among study groups
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