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. 2015 Mar 19;10(3):e0116770.
doi: 10.1371/journal.pone.0116770. eCollection 2015.

Easy to use plastic optical fiber-based biosensor for detection of butanal

Nunzio Cennamo  1 , Stefano Di Giovanni  2 , Antonio Varriale  2 , Maria Staiano  2 , Fabio Di Pietrantonio  3 , Andrea Notargiacomo  4 , Luigi Zeni  1 , Sabato D'Auria  5
Affiliations

Easy to use plastic optical fiber-based biosensor for detection of butanal

Nunzio Cennamo et al. PLoS One. .

Abstract

The final goal of this work is to achieve a selective detection of butanal by the realization of a simple, small-size and low cost experimental approach. To this end, a porcine odorant-binding protein was used in connection with surface plasmon resonance transduction in a plastic optical fiber tool for the selective detection of butanal by a competitive assay. This allows to reduce the cost and the size of the sensing device and it offers the possibility to design a "Lab-on-a-chip" platform. The obtained results showed that this system approach is able to selectively detect the presence of butanal in the concentration range from 20 μM to 1000 μM.

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Conflict of interest statement

Competing Interests: The authors confirm that co-author Sabato D’Auria is a PLOS ONE Editorial Board member. This does not alter the authors’ adherence to PLOS ONE Editorial policies and criteria.

Figures

Fig 1
Fig 1. Optical biosensor based on SPR in a POF and experimental setup.
Scheme of synthesis of derivatized SAM on chip POF. The gold surface was treated sequentially with a solution of α-lipoic acid (a), ethylenediamine (b) and butyric acid (c).
Fig 2
Fig 2. Atomic force microscopy.
3D rendering of: a gold coated Si substrate a) before and b) after functionalization with lipoic acid, and c) after additional subsequent functionalization with butyric acid and pOBP. Note that a different Z scale was used for the three images.
Fig 3
Fig 3. Porcine Odorant Binding Protein structure.
Cartoon representation of the structure pOBP with butanal. The image was produced by Protein workshop Right.
Fig 4
Fig 4. Binding experiment-SPR results.
(a) SPR transmission spectra, normalized to the air spectrum, for different pOBP concentrations in the case of sensor with gold layer with bio-receptor. Inset: Zoom of the resonance wavelengths region. (b) Resonance wavelength variation as a function of pOBP concentrations [μM].
Fig 5
Fig 5. Control—SPR experimental results.
SPR transmission spectra, normalized to the air spectrum, for different pOBP concentrations in the case of the reference sensor with gold layer—SAM without bio-receptor. Inset: Zoom of the resonance wavelengths region.
Fig 6
Fig 6. Competitive assay-SPR.
(a) SPR transmission spectra, normalized to the air spectrum, for different concentration in solution of butanal and with a fixed concentrations of pOBP (1.27 μM), in the case of sensor with gold layer with bio-receptor. Inset: Zoom of the resonance wavelengths region. (b) Resonance wavelength variation as a function of butanal concentration, obtained with binding of a fixed concentration of pOBP in presence of increasing concentration of butanal during pre-incubation time.
See this image and copyright information in PMC

References

    1. Beránek J, Kubátová A (2008) Evaluation of solid-phase microextraction methods for determination of trace concentration aldehydes in aqueous solution. Journal of Chromatography A 1209: 44–54. 10.1016/j.chroma.2008.09.013 - DOI - PubMed
    1. McGregor D, Bolt H, Cogliano V, Richter-Reichhelm HB (2006) Formaldehyde and glutaraldehyde and nasal cytotoxicity: case study within the context of the 2006 IPCS Human Framework for the Analysis of a cancer mode of action for humans. Crit Rev Toxicol 36: 821 - PubMed
    1. Schmarr HG, Potouridis T, Gan S, Sang W, Kopp B, et al. (2008) Analysis of carbonyl compounds via headspace solid-phase microextraction with on-fiber derivatization and gas chromatographic-ion trap tandem mass spectrometric determination of their O-(2,3,4,5,6-pentafluorobenzyl)oxime derivatives. Anal Chim Acta 617: 119–131. 10.1016/j.aca.2008.02.002 - DOI - PubMed
    1. Deng CH, Zhang XM (2004) A simple, rapid and sensitive method for determination of aldehydes in human blood by gas chromatography/mass spectrometry and solid-phase microextraction with on-fiber derivatization. Rapid Commun Mass Spectrom 18: 1715–1720. - PubMed
    1. Abbas A, Linman MJ, Cheng Q (2011) New trends in instrumental design for surface plasmon resonance-based biosensors. Biosens Bioelectron 26: 1815–1824. 10.1016/j.bios.2010.09.030 - DOI - PMC - PubMed

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