doi: 10.1016/0022-1759(85)90307-2.
Immunoenzymatic analysis by monoclonal antibodies of bacterial lipopolysaccharides after transfer to nitrocellulose
- PMID: 2579158
- DOI: 10.1016/0022-1759(85)90307-2
Item in Clipboard
Immunoenzymatic analysis by monoclonal antibodies of bacterial lipopolysaccharides after transfer to nitrocellulose
J Immunol Methods.
.
Abstract
A rapid, sensitive immunoenzymatic technique for the analysis of lipopolysaccharide (LPS) from gram-negative bacteria using monoclonal antibodies is described. After separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the LPS was either stained with silver or electrophoretically transferred to nitrocellulose. After reaction with anti-LPS monoclonal antibodies, the transferred antigens were visualized by reaction with alkaline-phosphatase-labelled anti-mouse antibodies and a substrate containing naphthol phosphoric acid and Fast Red.
Similar articles
-
Rapid serological analysis of bacterial lipopolysaccharides by electrotransfer to nitrocellulose.J Immunol Methods. 1985 Dec 27;85(2):371-82. doi: 10.1016/0022-1759(85)90146-2. J Immunol Methods. 1985. PMID: 3908569
-
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and monoclonal antibodies as tools for the subgrouping of Escherichia coli lipopolysaccharide O18 and O23 antigens.Infect Immun. 1986 Jan;51(1):286-93. doi: 10.1128/iai.51.1.286-293.1986. Infect Immun. 1986. PMID: 3510172 Free PMC article.
-
Immunoprotective murine monoclonal antibodies specific for the outer-core polysaccharide and for the O-antigen of Escherichia coli 0111:B4 lipopolysaccharide (LPS).J Immunol. 1987 Jul 15;139(2):557-61. J Immunol. 1987. PMID: 3298431
-
Antibodies to lipopolysaccharide.J Immunol Methods. 1995 Oct 12;186(1):1-15. doi: 10.1016/0022-1759(95)00123-r. J Immunol Methods. 1995. PMID: 7561138 Review.
-
Serogroup and serotype classification of bacterial pathogens.Methods Enzymol. 1994;235:159-74. doi: 10.1016/0076-6879(94)35138-4. Methods Enzymol. 1994. PMID: 8057893 Review. No abstract available.
Cited by
-
Subunit structure of the variable V-1 antigen of Mycoplasma pulmonis.Infect Immun. 1989 Jun;57(6):1684-90. doi: 10.1128/iai.57.6.1684-1690.1989. Infect Immun. 1989. PMID: 2722235 Free PMC article.
-
Purification and immunological characterization of a major low-molecular-weight lipoprotein from Borrelia burgdorferi.Infect Immun. 1992 Dec;60(12):4995-5003. doi: 10.1128/iai.60.12.4995-5003.1992. Infect Immun. 1992. PMID: 1452330 Free PMC article.
-
Dot-immunobinding assay with a monoclonal antibody for detection of group B meningococcal antigen.Eur J Clin Microbiol. 1986 Feb;5(1):44-6. doi: 10.1007/BF02013461. Eur J Clin Microbiol. 1986. PMID: 3084244 No abstract available.
-
A murine monoclonal antibody defines a unique epitope shared by Klebsiella lipopolysaccharides.Infect Immun. 1994 Apr;62(4):1282-8. doi: 10.1128/iai.62.4.1282-1288.1994. Infect Immun. 1994. PMID: 7510667 Free PMC article.
-
Analysis of C3 deposition and degradation on Neisseria meningitidis and Neisseria gonorrhoeae.Infect Immun. 1994 May;62(5):1755-60. doi: 10.1128/iai.62.5.1755-1760.1994. Infect Immun. 1994. PMID: 8168937 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
