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. 2015 Mar 11:10:1977-83.
doi: 10.2147/IJN.S79106. eCollection 2015.

Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects

Adithan Aravinthan  1 , Muthusamy Govarthanan  2 , Kandasamy Selvam  3 , Loganathan Praburaman  2 , Thangasamy Selvankumar  4 , Rangachari Balamurugan  1 , Seralathan Kamala-Kannan  5 , Jong-Hoon Kim  1
Affiliations

Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects

Adithan Aravinthan et al. Int J Nanomedicine. .

Abstract

A rapid, green phytosynthesis of silver nanoparticles (AgNPs) using the aqueous extract of Helianthus tuberosus (sunroot tuber) was reported in this study. The morphology of the AgNPs was determined by transmission electron microscopy (TEM). Scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS) and X-ray powder diffraction (XRD) analysis confirmed the presence of AgNPs. Fourier transform infrared spectroscopy (FTIR) analysis revealed that biomolecules in the tuber extract were involved in the reduction and capping of AgNPs. The energy-dispersive spectroscopy (EDS) analysis of the AgNPs, using an energy range of 2-4 keV, confirmed the presence of elemental silver without any contamination. Further, the synthesized AgNPs were evaluated against phytopathogens such as Ralstonia solanacearum and Xanthomonas axonopodis. The AgNPs (1-4 mM) extensively reduced the growth rate of the phytopathogens. In addition, the cytotoxic effect of the synthesized AgNPs was analyzed using rat splenocytes. The cell viability was decreased according to the increasing concentration of AgNPs and 67% of cell death was observed at 100 μg/mL.

Keywords: Helianthus tuberosus; cytotoxicity; nanobiotechnology; phytosynthesis; splenocytes.

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Figures

Figure 1
Figure 1
UV–Vis absorption spectrum of the AgNPs prepared from 1 mM AgNO3 solution. Abbreviation: AgNPs, silver nanoparticles.
Figure 2
Figure 2
TEM image of AgNPs. Abbreviations: TEM, transmission electron microscopy; AgNPs, silver nanoparticles.
Figure 3
Figure 3
SEM–EDS spectrum of AgNPs. A strong peak at 3 keV confirms the presence of Ag. Abbreviations: SEM–EDS, scanning electron microscopy–energy-dispersive spectroscopy; AgNPs, silver nanoparticles.
Figure 4
Figure 4
XRD pattern of AgNPs synthesized using tuber extract. Abbreviations: XRD, X-ray powder diffraction; AgNPs, silver nanoparticles.
Figure 5
Figure 5
FTIR spectra of AgNPs synthesized using tuber extract. Abbreviations: FTIR, Fourier transform infrared spectroscopy; AgNPs, silver nanoparticles.
Figure 6
Figure 6
Effect of AgNPs on growth of phytopathogenic bacteria. Notes: (A) Ralstonia solanacearum and (B) Xanthomonas axonopodis. Abbreviation: AgNPs, silver nanoparticles.
Figure 7
Figure 7
Cytotoxic effect of AgNPs on growth of rat splenocytes. Notes: (A) a 96-well plate image of MTT assay, the toxicity is indicated by color reduction; (B) dose dependent reduction of cell viability at 595 nm. *P<0.05; **P<0.01; ***P<0.001. Abbreviation: AgNPs, silver nanoparticles.
See this image and copyright information in PMC

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