Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 2000;3(1):13-6.
doi: 10.1298/jjpta.3.13.

The effect of low calcium diet on bone in ovariectomized mice

A Minematsu  1 , O Yoshimura  1 , H Yotsuji  2 , H Ichigo  2 , K Takayanagi  1 , R Kobayashi  1 , M Hosoda  1 , H Sasaki  1 , H Maejima  1 , Y Matsuda  1 , S Tanaka  1 , N Kanemura  1 , A Matsuo  1
Affiliations
Case Reports

The effect of low calcium diet on bone in ovariectomized mice

A Minematsu et al. J Jpn Phys Ther Assoc. 2000.

Abstract

This study investigates the effects of Ca on bone in the ovariectomized mice. Twenty-six female ICR mice aged 5 weeks were used. They were ovariectomized (OVX) or sham-operated (SHAM) and fed standard mouse diet (SF) or special low calcium diet (L.Ca), respectively. All animals were sacrificed at day 100 after operation. Mechanical strength of the left femur and tibia was measured by the three-point bending strength test. The bones were dried, weighed and burned to ash. Correlation between mechanical strength and ash content was found. A specimen of the right tibia was prepared. Mechanical strength, ash content and ratio of dry bone weight to body weight of the femur and tibia in OVX and L.Ca mice were significantly less than in SHAM and SF mice. SHAM/SF mice and OVX/L.Ca group showed highest and lowest values in all cases. The values for the femur and tibia in OVX/SF mice were lower than in SHAM/SF group and in OVX/L.Ca group were less than in OVX/SF mice. Correlation coefficients for mechanical strength and ash content were 0.704 and 0.776 for the femur and tibia. Ca is thus related to inhibition of bone loss and maintenance of bone mass and effective prevention of osteoporosis.

Keywords: ash content; low calcium diet; mechanical strength; ovariectomized mice.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
The proximal tibia of OVX/SF (A) group. Trabecular is remained barely but a gap is remarkable.
Fig. 2
Fig. 2
The proximal tibia of SHAM/SF (B) group. Trabecular is dense and a gap is not found.
Fig. 3
Fig. 3
The proximal tibia of OVX/L.Ca (C) group. There are few trabecular and this area becomes a hollow.
Fig. 4
Fig. 4
The proximal tibia of SHAM/L.Ca (D) group. Trabecular is sparse and a gap is found here and there.
See this image and copyright information in PMC

References

    1. Bonjor J, et al. : Critical years and stages of puberty for spinal and femoral bone mass accumulation during adolescence. J Clin Endocrinol Metab 73: 555-563, 1991. - PubMed
    1. Nordin BEC, Wilkinson R, et al. : Calcium absorption in the elderly. Calcif Tissue Res 21: 442-447, 1976. - PubMed
    1. Matkovic V, et al. : Bone status and fracture rates in two regions of Yugoslavia. Am J Clin Nutr 32: 540, 1979. - PubMed
    1. Comming RG: Calcium intake and bone mass: quantitative review of the evidence. Calcif Tissue Int 47: 194-201, 1990. - PubMed
    1. Riis B, et al. : Does calcium supplementation prevent postmenopausal bone loss? —A double-blind, controlled clinical study—. N Engl J Med 316: 173, 1987. - PubMed

Publication types