In Vitro, Pharmacokinetic, Pharmacodynamic, and Safety Comparisons of Single and Combined Administration of Tiotropium and Salmeterol in COPD Patients Using Different Dry Powder Inhalers

Abstract

In vitro Andersen cascade impactor-sized mass (ISM) and aerodynamic fine particle mass (FPM) <5 μm for tiotropium and salmeterol combined in a novel inhalation powder formulation containing 7.5 μg tiotropium/25 μg salmeterol (TSHH) were similar (within ±15%) to reference products containing 18 μg of tiotropium (Spiriva® HandiHaler®) (TioHH) and 50 μg of salmeterol (Serevent® Diskus®) (SalD). The pharmacokinetics (PK), pharmacodynamics, safety, and tolerability of the novel fixed-dose TSHH formulation administered once daily was compared with the single-agent therapies TioHH (once daily [qd]) and SalD (twice daily [bid]) and with the jointly administered combination of TioHH (qd) plus SalD (bid) in a randomized, 22-week, open-label, four-way crossover study in 50 patients with chronic obstructive pulmonary disease (COPD). For tiotropium, TSHH and TioHH were bioequivalent based on mean steady-state plasma area under the plasma concentration-time curves (AUC), while the urinary excretion amount was higher for TSHH and not bioequivalent to TioHH. Tiotropium peak plasma concentrations at steady state (C max,ss) were 40% higher with TSHH. For salmeterol, substantial differences were observed in plasma AUCs and Cmax,ss. No significant differences in 8-h forced expiratory volume in 1 s or forced vital capacity were detected for the TSHH (qd) against the combination of TioHH (qd) with SalD (bid). Maintenance therapy with tiotropium plus salmeterol as TSHH or as the jointly administered reference products is superior to either agent alone, safe, and well tolerated in COPD patients. In vitro results were not predictive of clinical PK findings for both tiotropium and salmeterol for the TSHH dry powder inhaler product.

Fig. 1

Aerodynamic particle size profiles for the tiotropium treatments. Testing was performed at 39 L/min. Individual points represent the pooled average of 24 capsules for TioHH and 12 capsules for TSHH

Fig. 2

Comparison of aerodynamic particle size profiles for the salmeterol treatments. SalD was tested at 70 L/min. TSHH was tested at 39 L/min. Individual points represent the pooled average of 12 capsules for TSHH and 9 actuations for SalD. Impactor-sized mass and fine particle mass results are overlapping data points. I/T/P inlet + throat + pre-separator, ISM impactor-sized mass, FPM fine particle mass <5 μm

Fig. 3

Comparison of salmeterol aerodynamic particle size profiles for TSHH and SalD at 39 L/min. Individual points represent the pooled average of 12 capsules for TSHH and 6 actuations for SalD

Fig. 4

Comparison of mean tiotropium plasma concentration-time profiles. Tiotropium concentrations for TioHH and TioHH + SalD were below limit of quantitation after 4 h

Fig. 5

Comparison of mean tiotropium cumulative urinary excretion profiles

Fig. 6

Comparison of mean salmeterol plasma concentration–time profiles

Fig. 7

Comparison of mean 8-h FEV₁ (upper panel) and 8-h FVC (lower panel) profiles for the four study treatments