The power of the yeast two-hybrid system in the identification of novel drug targets: building and modulating PPP1 interactomes

Abstract

Since the description of the yeast two-hybrid (Y2H) method, it has become more and more evident that it is the most commonly used method to identify protein-protein interactions (PPIs). The improvements in the original Y2H methodology in parallel with the idea that PPIs are promising drug targets, offer an excellent opportunity to apply the principles of this molecular biology technique to the pharmaceutical field. Additionally, the theoretical developments in the networks field make PPI networks very useful frameworks that facilitate many discoveries in biomedicine. This review highlights the relevance of Y2H in the determination of PPIs, specifically phosphoprotein phosphatase 1 interactions, and its possible outcomes in pharmaceutical research.

Keywords: drug screening; drug targets; interactomes; molecular interactions; phosphoprotein phosphatase 1; protein–protein interactions; yeast two-hybrid variants.