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. 2015 Jun:113:10-2.
doi: 10.1016/j.mimet.2015.03.018. Epub 2015 Mar 21.

It's a dirty job--A robust method for the purification and de novo genome assembly of Cryptosporidium from clinical material

Sofia Andersson  1 , Per Sikora  1 , Maria L Karlberg  1 , Jadwiga Winiecka-Krusnell  1 , Erik Alm  1 , Jessica Beser  1 , Romanico B G Arrighi  2
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It's a dirty job--A robust method for the purification and de novo genome assembly of Cryptosporidium from clinical material

Sofia Andersson et al. J Microbiol Methods. 2015 Jun.

Abstract

We have developed a novel strategy for the purification of Cryptosporidium oocysts from clinical samples using IMS and PCR amplification of target DNA to facilitate uniform coverage genome sequencing and de novo assembly. Our procedure could also be used for other microbial pathogens from clinical specimens.

Keywords: Clinical parasitology; Cryptosporidium; Genome sequencing; Immunomagnetic separation.

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