[Bio-molecular evaluation of human transcriptomic profile in whole blood sample: a potential clinical approach?]

Abstract

At present, the analysis of transcriptional profile of blood samples is the more efficient and promising approach to study diagnostic and prognostic biomarkers, complementing the quantitative information obtained by a simple plasma assay, with the qualitative one of mRNA expression. More novel and available blood collection techniques are being developed reducing problems linked to the invasive bioptic approach. To this purpose, in the last years, methods able to isolate leukocytes from minimal quantity of whole blood have been developed, among these the PAXgene methods offer undeniable advantages. This latter method allows to study the transcriptomic profile of many biomarkers and related pathways involved in different pathologies, until now analyzed only with molecular biology techniques at tissue level, identifying new and more specific therapeutic targets.