Nucleotide sequence cleavages of manganese-bleomycin induced by reductant, hydrogen peroxide and ultraviolet light. Comparison with iron- and cobalt-bleomycins

Abstract

The prominent DNA breakages of bleomycin-Mn complex system were induced by reductant, hydrogen peroxide and ultraviolet light, and these three induction systems gave remarkably similar nucleotide sequence cleavage modes. The preferred DNA cleavage sites at guanine-cytosine(5'----3') and guanine-thymine(5'----3') sequences were appreciably comparable to those of the corresponding bleomycin-Fe complex systems, but not identical. In contrast, the bleomycin-Co complex system significantly degraded isolated DNA only by irradiation of ultraviolet light. The present results provide valuable information on the role of transition metals on DNA cleavage reaction of bleomycin.