Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Apr;38(4):409-16.
doi: 10.1136/jcp.38.4.409.

Effect of neuraminidase on the expression of the 3-fucosyl-N-acetyllactosamine antigen in human tissues

Effect of neuraminidase on the expression of the 3-fucosyl-N-acetyllactosamine antigen in human tissues

A J Howie et al. J Clin Pathol. 1985 Apr.

Abstract

The monoclonal antibody AGF4.48 detects the 3-fucosyl-N-acetyllactosamine carbohydrate structure and reacts with a variety of human tissues, as shown by an immunoperoxidase method on paraffin sections of formalin fixed material. The intensity and distribution of the immunoperoxidase reactivity were affected by treatment of sections with neuraminidase. In some sites, such as bone marrow, brain, breast, and pituitary, the reaction was more intense after neuraminidase treatment. In several organs, including kidney, oesophagus, pancreas, submandibular gland, and uterus, the reaction was more widespread after neuraminidase treatment. Other organs, such as liver, lung, lymph node, skin, and uterine tube, showed an altered distribution of reactivity with AGF4.48 after sections were treated with neuraminidase. These results show that the 3-fucosyl-N-acetyllactosamine antigen is cryptic in many tissues. The antigen is masked to a variable extent in different tissues by neuraminic acid residues and can be revealed by the simple procedure of pretreating sections with neuraminidase. The value of using AGF4.48 antibody in combination with the enzyme neuraminidase to show accessible and cryptic antigen is that this reveals distinctive patterns of sialylation within various normal tissues. Whether similar patterns occur in disease will be of particular interest.

PubMed Disclaimer

References

    1. J Biol Chem. 1969 Oct 25;244(20):5496-502 - PubMed
    1. FEBS Lett. 1976 Feb 15;62(2):161-4 - PubMed
    1. Nature. 1981 Jul 9;292(5819):156-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 May;78(5):3039-43 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4515-9 - PubMed

Publication types

LinkOut - more resources