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. 2015 Mar 30:5:9418.
doi: 10.1038/srep09418.

In-line phase-contrast and grating-based phase-contrast synchrotron imaging study of brain micrometastasis of breast cancer

Sheng Huang  1 , Binquan Kou  2 , Yayun Chi  1 , Yan Xi  3 , Yixin Cao  2 , Wenli Cui  4 , Xin Hu  1 , Zhimin Shao  1 , Han Guo  5 , Yanan Fu  5 , Tiqiao Xiao  5 , Jianqi Sun  6 , Jun Zhao  3 , Yujie Wang  2 , Jiong Wu  1
Affiliations

In-line phase-contrast and grating-based phase-contrast synchrotron imaging study of brain micrometastasis of breast cancer

Sheng Huang et al. Sci Rep. .

Abstract

Current bio-medical imaging researches aim to detect brain micrometastasis in early stage for its increasing incidence and high mortality rates. Synchrotron phase-contrast imaging techniques, such as in-line phase-contrast (IPC) and grating-based phase-contrast (GPC) imaging, could provide a high spatial and density imaging study of biological specimens' 3D structures. In this study, we demonstrated the detection efficiencies of these two imaging tools on breast cancer micrometastasis in an ex vivo mouse brain. We found that both IPC and GPC can differentiate abnormal brain structures induced by micrometastasis from the surrounding normal tissues. We also found that GPC was more sensitive in detecting the small metastasis as compared to IPC.

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Figures

Figure 1
Figure 1. The brain slice and reconstructed tomogram in three formats, including IPC-CT (c) and GPC-CT (e), as well as H&E pathological graphs (a) in motor cortex metastasis.
Enlargement of the motor cortex metastasis in (b) H&E pathological graph, (d) IPC-CT image and (f) GPC-CT (×4.3, 1170 × 790 μm2). (g) The δ values along the red line in Fig. 1d. (h) The δ values along the blue line in Fig. 1f. Scale bar: 500 μm (a, c, e) and 200 μm (b, d, f).
Figure 2
Figure 2. The brain slice and reconstructed tomogram in three formats, including IPC-CT (c) and GPC-CT (e), as well as H&E pathological graphs (a) in hippocampus metastasis.
Enlargement of the hippocampus metastasis in (b) H&E pathological graph, (d) IPC-CT image and (f) GPC-CT image (×5.7, 700 × 580 μm2). (g) The δ values along the red line in Fig. 2d. (h) The δ values along the blue line in Fig. 2f. Scale bar: 500 μm (a, c, e) and 200 μm (b, d, f).
Figure 3
Figure 3. Three regions selected in IPC-CT image (a, b) and GPC-CT iamge (c, d) for CNR analysis.
(1) red square: tumor (2) yellow square: surrounding region of the tumor (3) blue square: background region. The areas of the square regions in (a), (c) are 200 × 200 μm2 while 120 × 120 μm2 in (b), (d). Scale bar: 500 μm.
Figure 4
Figure 4. The reconstructed mouse brain structure by GPC tomography.
Breast cancer metastatic foci in motor cortex and hippocampus are marked in pink.
See this image and copyright information in PMC

References

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