Hb F-Tokyo or alpha 2G gamma 2 34(B16)Val----Ile, a silent gamma chain variant detected by reverse phase high performance liquid chromatography

Abstract

Hb F-Tokyo with a Valine----Isoleucine replacement at position gamma 34(B16) is a G gamma chain variant which was discovered by reverse phase chromatography as this method permitted the nearly complete separation of the three types of gamma chain. The chemical characterization was greatly facilitated by the use of a larger, preparative, HPLC column which allowed the isolation of sufficient quantities of the different gamma chains.