Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015:2015:532386.
doi: 10.1155/2015/532386. Epub 2015 Mar 3.

Identification of Putative Molecular Markers Associated with Root Traits in Coffea canephora Pierre ex Froehner

Devaraja Achar  1 , Mallikarjuana G Awati  2 , M Udayakumar  1 , T G Prasad  1
Affiliations

Identification of Putative Molecular Markers Associated with Root Traits in Coffea canephora Pierre ex Froehner

Devaraja Achar et al. Mol Biol Int. 2015.

Abstract

Coffea canephora exhibit poor root system and are very sensitive to drought stress that affects growth and production. Deeper root system has been largely empirical as better avoidance to soil water limitation in drought condition. The present study aimed to identify molecular markers linked to high root types in Coffea canephora using molecular markers. Contrasting parents, L1 valley with low root and S.3334 with high root type, were crossed, and 134 F1 individuals were phenotyped for root and associated physiological traits (29 traits) and genotyped with 41 of the 320 RAPD and 9 of the 55 SSR polymorphic primers. Single marker analysis was deployed for detecting the association of markers linked to root associated traits by SAS software. There were 13 putative RAPD markers associated with root traits such as root length, secondary roots, root dry weight, and root to shoot ratio, in which root length associated marker OPS1850 showed high phenotypic variance of 6.86%. Two microsatellite markers linked to root length (CPCM13400) and root to shoot ratio (CM211300). Besides, 25 markers were associated with more than one trait and few of the markers were associated with positively related physiological traits and can be used in marker assisted trait selection.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Genetic variability of root system and quantitative traits of mapping population of L1 valley × S.334.
Figure 2
Figure 2
Relationship between the RDW/TDM, RDW/CWT, TDM/CWT, CWT/MTR, CWT/MTR, and WUE/Δ13C in the mapping population of C. canephora (L1 valley × S.334).
See this image and copyright information in PMC

References

    1. DaMatta F. M. Exploring drought tolerance in coffee: a physiological approach with some insights for plant breeding. Brazilian Journal of Plant Physiology. 2004;16(1):1–6.
    1. Nesbitt M. The Cultural History of Plants. Taylor & Francis; 2005.
    1. Damatta F. M. Drought as a multidimensional stress affecting photosynthesis in tropical tree crops. In: Hemantaranjan A., editor. Advances in Plant Physiology. Vol. 5. 2003. pp. 227–265.
    1. Harovon Mogel K. Genotype × environment × management: interactions key to beating future droughts. CSA News. 2013;58:4–9. doi: 10.2134/csa2013-58-2-1. - DOI
    1. DaMatta F. M., Chaves A. R. M., Pinheiro H. A., Ducatti C., Loureiro M. E. Drought tolerance of two field-grown clones of Coffea canephora . Plant Science. 2003;164(1):111–117. doi: 10.1016/s0168-9452(02)00342-4. - DOI

LinkOut - more resources