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. 2015 Mar 26;16(4):6855-67.
doi: 10.3390/ijms16046855.

Resistance training regulates cardiac function through modulation of miRNA-214

Stéphano Freitas Soares Melo  1 , Valério Garrone Barauna  2 , Miguel Araújo Carneiro Júnior  3 , Luiz Henrique Marchesi Bozi  4 , Lucas Rios Drummond  5 , Antônio José Natali  6 , Edilamar Menezes de Oliveira  7
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Resistance training regulates cardiac function through modulation of miRNA-214

Stéphano Freitas Soares Melo et al. Int J Mol Sci. .

Abstract

Aims: To determine the effects of resistance training (RT) on the expression of microRNA (miRNA)-214 and its target in sarcoplasmic reticulum Ca2+-ATPase (SERCA2a), and on the morphological and mechanical properties of isolated left ventricular myocytes.

Main methods: Male Wistar rats were divided into two groups (n = 7/group): Control (CO) or trained (TR). The exercise-training protocol consisted of: 4 × 12 bouts, 5×/week during 8 weeks, with 80% of one repetition maximum.

Key findings: RT increased the left ventricular myocyte width by 15% and volume by 12%, compared with control animals (p < 0.05). The time to half relaxation and time to peak were 8.4% and 4.4% lower, respectively, in cells from TR group as compared to CO group (p < 0.05). RT decreased miRNA-214 level by 18.5% while its target SERCA2a expression were 18.5% higher (p < 0.05).

Significance: Our findings showed that RT increases single left ventricular myocyte dimensions and also leads to faster cell contraction and relaxation. These mechanical adaptations may be related to the augmented expression of SERCA2a which, in turn, may be associated with the epigenetic modification of decreased miRNA-214 expression.

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Figures

Figure 1
Figure 1
Cardiomyocyte dimensions of isolated LV myocytes. (A) Cell width; (B) Cell volume; and (C) Cell length. Results are presented as mean ± standard deviation of 70 cells per group and 7 animals each group. At least 10 cells were analyzed from each animal. * p < 0.05 when compared to control group.
Figure 2
Figure 2
Cell contractility (A) Time to half relaxation; and (B) Time to peak contraction of cardiomyocytes from trained (n = 7 rats and 108 cells) and control (n = 7 rats and 96 cells) groups. Results are presented as mean ± standard deviation. * p < 0.05 when compared to control group.
Figure 3
Figure 3
Protein expression level and miRNA. (A) Representative western blot image and SERCA2a protein expression; (B) miRNA expression level; and (C) Schematic summary of the results showing both the morphological and functional adaptations induced by the resistance training. Results are presented as mean ± standard deviation. * p < 0.05 when compared to control group.
Figure 4
Figure 4
Apparatus adapted from Tamaki et al. (1992) [50], used to perform resistance training in the rats [3,4].
See this image and copyright information in PMC

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