doi: 10.1093/nar/13.1.45.
Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides
- PMID: 2582352
- PMCID: PMC340973
- DOI: 10.1093/nar/13.1.45
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Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides
Nucleic Acids Res.
.
Free PMC article
Abstract
Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-labeled oligonucleotide probes were studied.
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