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. 1985 Jan 11;13(1):45-57.
doi: 10.1093/nar/13.1.45.

Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides

T Kempe, W I Sundquist, F Chow, S L Hu
Free PMC article

Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides

T Kempe et al. Nucleic Acids Res. .
Free PMC article

Abstract

Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-labeled oligonucleotide probes were studied.

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References

    1. Methods Enzymol. 1974;34:265-7 - PubMed
    1. DNA. 1984 Jun;3(3):269-77 - PubMed
    1. Biochemistry. 1975 Mar 25;14(6):1225-9 - PubMed
    1. Chromosoma. 1975 Nov 24;53(2):107-17 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed