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. 2015 Apr;15(2):160-7.
doi: 10.7861/clinmedicine.15-2-160.

Harveian Oration 2014: Stem cells and cell replacement prospects

John Gurdon  1
Affiliations

Harveian Oration 2014: Stem cells and cell replacement prospects

John Gurdon. Clin Med (Lond). 2015 Apr.
No abstract available

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Figures

Fig 1.
Fig 1.
Single cell nuclear transfer for (A) therapeutic and (B) reproductive cloning.
Fig 2.
Fig 2.
Xenopus egg. The egg is surrounded by a thick layer of jelly that is impenetrable by needles.
Fig 3.
Fig 3.
Microinjection needles (A) before microforging (B) after microforging.
Fig 4.
Fig 4.
Intestinal tract of feeding tadpole (GFP marked).
Fig 5.
Fig 5.
Serial nuclear transplantation. Wakayama et al (2013) demonstrated 25 generations of serial nuclear transfer, with 500 cell divisions from the first donor nucleus. No cancer incidence was observed.
Fig 6.
Fig 6.
Mechanisms of cell differentiation. The first mechanism is asymmetric distribution of determinants in the egg, giving rise to an asymmetric distribution of cells in the embryo. Subsequently, signalling between cells (red arrows) causes further differentiation to give the ectoderm, mesoderm and endoderm.
Fig 7.
Fig 7.
Formation of specialised cells from stem cells. Gene activation molecules accumulate in the stem cell and move to one side in response to an external signal. Asymmetric cell division gives rise to a specialised skin cell and depends on the concentration of transcription factors (red spots).
Fig 8.
Fig 8.
Cells specialise in ways determined by the concentration of morphogen molecules (pink circles) that come from the source and reach distant cells by diffusion.
Fig 9.
Fig 9.
Time course of transcriptional activation of transplanted somatic cell nuclei by oocytes.
See this image and copyright information in PMC

References

    1. Weismann A. Das Keimplasma, eine theorie der Vererbung. In: Parker WN, Ronnfeldt H. (eds), The germ-plasm: a theory of heredity. London: Walter Scott, 1892.
    1. Briggs R, King TJ. Transplantation of living nuclei from blastula cells into enucleated frogs’ eggs. Proc Nat Acad Sci 1952;38:455–63. - PMC - PubMed
    1. Gurdon JB, Uehlinger V. ‘Fertile’ intestine nuclei. Nature 1966;210:1240–1. - PubMed
    1. Wakayama S, Kohda T, Obokata H, et al. Successful serial recloning in the mouse over multiple generations. Cell Stem Cell 2013;12:293–7. - PubMed
    1. Levi-Montalcini R. The nerve growth factor: thirty-five years later. EMBO J 1987;6:1145–54. - PMC - PubMed