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. 2015 May 4;16(7):1115-9.
doi: 10.1002/cbic.201500094. Epub 2015 Mar 31.

Simple "on-demand" production of bioactive natural products

Edna Bode  1 , Alexander O Brachmann, Carsten Kegler, Rukayye Simsek, Christina Dauth, Qiuqin Zhou, Marcel Kaiser, Petra Klemmt, Helge B Bode
Affiliations

Simple "on-demand" production of bioactive natural products

Edna Bode et al. Chembiochem. .

Abstract

Exchange of the native promoter to the arabinose-inducible promoter PBAD was established in entomopathogenic bacteria to silence and/or activate gene clusters involved in natural product biosynthesis. This allowed the "on-demand" production of GameXPeptides, xenoamicins, and the blue pigment indigoidine. The gene clusters for the novel "mevalagmapeptides" and the highly toxic xenorhabdins were identified by this approach.

Keywords: biosynthesis; natural products; overproduction; promoter-exchange approach; “silent” gene cluster.

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