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. 2015 Apr-Jun;7(2):193-7.
doi: 10.4103/0974-8490.150541.

A study of Nigella sativa induced growth inhibition of MCF and HepG2 cell lines: An anti-neoplastic study along with its mechanism of action

Y Padmanabha Reddy  1 , K B Chandrasekhar  2 , Mohammed Jaffar Sadiq  3
Affiliations

A study of Nigella sativa induced growth inhibition of MCF and HepG2 cell lines: An anti-neoplastic study along with its mechanism of action

Y Padmanabha Reddy et al. Pharmacognosy Res. 2015 Apr-Jun.

Abstract

Objective: To evaluate the anticancer potential of seeds of Nigella sativa using MCF and HepG2 cell lines along with its mechanism of action.

Materials and methods: (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and acridine orange/ethidium bromide nuclear staining technique were selected to evaluate anticancer potential and mechanism of action of test extract.

Results: Aqueous extract of N.sativa at a test dose of 180 mg and 300 mg was identified to be the best as anticancer agent against MCF and HepG2 cell lines among different solvent test extract where doxorubicin and cisplatin were employed as standard references.

Discussion: Further study including separation and characterization of active principles in the aqueous extract shall prove beneficial.

Keywords: Anti-neoplastic; HepG2; Nigella sativa seeds; Nuclear staining; human breast adenocarcinoma cells.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
MCF cells without standard or test substances
Figure 2
Figure 2
Cisplatin treated (standard drug)
Figure 3
Figure 3
Autonomic nervous system treated (test drug)
Figure 4
Figure 4
Comparative graphical representation of effect of standard and test drug on the MCF cell lines
See this image and copyright information in PMC

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