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. 2015 Mar 20:6:22.
doi: 10.4103/2008-7802.153537. eCollection 2015.

Preventive role of estradiol on kidney injury induced by renal ischemia-reperfusion in male and female rats

Akram Iran-Nejad  1 , Mehdi Nematbakhsh  2 , Fatemeh Eshraghi-Jazi  1 , Ardeshir Talebi  3
Affiliations

Preventive role of estradiol on kidney injury induced by renal ischemia-reperfusion in male and female rats

Akram Iran-Nejad et al. Int J Prev Med. .

Abstract

Background: Renal ischemia-reperfusion (RIR) is the main cause of renal failure. The incidence of RIR injury seems to be gender-related due to female sex hormone; estrogen. This study was designed to investigate the protective role of estrogen against RIR injury in male and ovariectomized female rats.

Methods: Thirty-nine Wistar rats were used in this study as male and ovariectomized female rats in the sham-operated, RIR, and estradiol-treated plus RIR groups. The RIR was induced by clamping the renal vessels for 45 min and then 24 h of reperfusion. All animals finally were sacrificed for the measurements.

Results: The serum levels of creatinine and blood urea nitrogen and kidney tissue damage score significantly increased in both male and female RIR rats (P < 0.05). Estradiol however significantly attenuated theses parameters (P < 0.05) toward normal levels in female (P < 0.05), but not in male rats. Kidney weight increased in both genders and estradiol intensified it in the male rats (P < 0.05). Uterus weight was increased by estradiol in female rats (P < 0.05) and testis weight did not alter in male rats.

Conclusions: Estradiol demonstrated a protective role against RIR injury in female rats; however, estradiol as an antioxidant could not protect the male kidney from RIR injury.

Keywords: Estrogen; gender; rat; renal ischemia-reperfusion.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Serum creatinine (Cr) and blood urea nitrogen (BUN) levels, testis weight (TW) and kidney weight (g)/100 g bodyweight (KW), kidney tissue damage score (KTDS) in the male groups. Abbreviations of Isch and Isch + Es were applied for ischemia and ischemia + estradiol. *and# indicates significant differences between the sham-operated and Isch groups, and between the Isch and Isch + estradiol groups, respectively
Figure 2
Figure 2
Serum levels of creatinine (Cr) and blood urea nitrogen (BUN) levels, uterus weight (UW) and kidney weight (g)/100 g body weight (KW), kidney tissue damage score (KTDS) in female groups. Abbreviations of Isch and Isch + Es were assigned to ischemia and ischemia + estradiol. *and# indicates significant differences between the sham-operated and Isch groups, and between Isch and Isch + estradiol groups, respectively
Figure 3
Figure 3
The pathology images (×100) of kidney tissue in the experimental groups. Abbreviations of Isch and Isch + Es were assigned to ischemia and ischemia + estradiol
See this image and copyright information in PMC

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