SOCS1 and SOCS3 are expressed in mononuclear cells in human cytomegalovirus viremia after allogeneic hematopoietic stem cell transplantation

Abstract

Background: The expression of the SOCS genes in cytomegalovirus (CMV) viremia after hematopoietic stem cell transplantation (HSCT) remains largely unexplored.

Methods: Using quantitative RT-PCR of mononuclear cells, we conducted pairwise comparison of SOCS1 and SOCS3 expression levels among a healthy donor group (N=55), a pre-HSCT group (N=17), and the recipient subgroup (N=107), which were divided according to the occurrence of CMV viremia and acute graft-versus-host disease (aGVHD).

Results: Compared to that in the healthy donor group, SOCS1 expression was higher in the CMV+ subgroup, especially in the CMV+GVHD- group, but decreased in the other subgroups. When compared to the expression in the pre-HSCT group, SOCS1 expression was significantly higher in the CMV+ subgroup, especially in the CMV+GVHD+ subgroup. Meanwhile, compared to that in the healthy donor group, SOCS3 expression was significantly lower in all other groups. The CMV-GVHD- subgroup showed significantly lower SOCS3 expression compared to the CMV+ subgroup, the CMV+GVHD+ subgroup, and the CMV+GVHD- subgroup.

Conclusion: We report differential expression of SOCS genes according to CMV viremia with acute GVHD occurrence after HSCT, suggesting that regulation of SOCS expression is associated with CMV viremia.

Keywords: Allogeneic HSCT; Cytomegalovirus (CMV) viremia; GVHD; Quantitative real-time PCR; Suppressor of cytokine signaling (SOCS) 1 and 3.

Fig. 1. Comparison of (A) SOCS1 and (B) SOCS3 gene expression levels in the healthy donor group, the pre-HSCT group, and the recipients. Mononuclear cells from recipients were isolated and subjected to real-time quantitative reverse transcription-polymerase chain reaction. Relative gene expression (RE) was normalized to that of ABL1. Error bars indicate±standard error.