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. 2015:1299:177-90.
doi: 10.1007/978-1-4939-2572-8_15.

Gene transfer into cardiac myocytes

Sarah E Lang  1 , Margaret V Westfall
Affiliations

Gene transfer into cardiac myocytes

Sarah E Lang et al. Methods Mol Biol. 2015.

Abstract

Traditional methods for DNA transfection are often inefficient and toxic for terminally differentiated cells, such as cardiac myocytes. Vector-based gene transfer is an efficient approach for introducing exogenous cDNA into these types of primary cell cultures. In this chapter, separate protocols for adult rat cardiac myocyte isolation and gene transfer with recombinant adenovirus are provided and are routinely utilized for studying the effects of sarcomeric proteins on myofilament function.

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References

    1. Davis J, et al. Designing heart performance by gene transfer. Physiol Rev. 2008;88(4):1567–1651. - PubMed
    1. Westfall MV, et al. Adenovirus-mediated myofilament gene transfer into adult cardiac myocytes. Methods Cell Biol. 1997;52:307–322. - PubMed
    1. Panchal RG, et al. Gene transfer: manipulating and monitoring function in cells and tissues. Clin Exp Pharmacol Physiol. 2001;28(8):687–691. - PubMed
    1. McWhinney CD, Hansen C, Robishaw JD. Alpha-1 adrenergic signaling in a cardiac murine atrial myocyte (HL-1) cell line. Mol Cell Biochem. 2000;214(1–2):111–119. - PubMed
    1. Beaumont J, et al. Overexpression of human truncated peroxisome proliferator-activated receptor alpha induces apoptosis in HL-1 cardiomyocytes. Cardiovasc Res. 2008;79(3):458–463. - PubMed

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