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. 2015 Mar 17:6:141.
doi: 10.3389/fpls.2015.00141. eCollection 2015.

Uncovering the genetic architecture of Colletotrichum lindemuthianum resistance through QTL mapping and epistatic interaction analysis in common bean

Ana M González  1 , Fernando J Yuste-Lisbona  2 , A Paula Rodiño  1 , Antonio M De Ron  1 , Carmen Capel  2 , Manuel García-Alcázar  2 , Rafael Lozano  2 , Marta Santalla  1
Affiliations

Uncovering the genetic architecture of Colletotrichum lindemuthianum resistance through QTL mapping and epistatic interaction analysis in common bean

Ana M González et al. Front Plant Sci. .

Abstract

Colletotrichum lindemuthianum is a hemibiotrophic fungal pathogen that causes anthracnose disease in common bean. Despite the genetics of anthracnose resistance has been studied for a long time, few quantitative trait loci (QTLs) studies have been conducted on this species. The present work examines the genetic basis of quantitative resistance to races 23 and 1545 of C. lindemuthianum in different organs (stem, leaf and petiole). A population of 185 recombinant inbred lines (RIL) derived from the cross PMB0225 × PHA1037 was evaluated for anthracnose resistance under natural and artificial photoperiod growth conditions. Using multi-environment QTL mapping approach, 10 and 16 main effect QTLs were identified for resistance to anthracnose races 23 and 1545, respectively. The homologous genomic regions corresponding to 17 of the 26 main effect QTLs detected were positive for the presence of resistance-associated gene cluster encoding nucleotide-binding and leucine-rich repeat (NL) proteins. Among them, it is worth noting that the main effect QTLs detected on linkage group 05 for resistance to race 1545 in stem, petiole and leaf were located within a 1.2 Mb region. The NL gene Phvul.005G117900 is located in this region, which can be considered an important candidate gene for the non-organ-specific QTL identified here. Furthermore, a total of 39 epistatic QTL (E-QTLs) (21 for resistance to race 23 and 18 for resistance to race 1545) involved in 20 epistatic interactions (eleven and nine interactions for resistance to races 23 and 1545, respectively) were identified. None of the main and epistatic QTLs detected displayed significant environment interaction effects. The present research provides essential information not only for the better understanding of the plant-pathogen interaction but also for the application of genomic assisted breeding for anthracnose resistance improvement in common bean through application of marker-assisted selection (MAS).

Keywords: NL genes; Phaseolus vulgaris L.; QTL; anthracnose; epistasis; resistance gene clusters.

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Figures

Figure 1
Figure 1
Anthracnose infection phenotypes in stem (A), leaf (B), and petiole (C). Scale bar 1 cm.
Figure 2
Figure 2
Location of main effect QTLs for resistance to anthracnose races 23 and 1545 on a genetic linkage map of common bean based on the RIL population developed from the cross PMB0225 × PHA1037. Distances among markers are indicated in cM to the left of the linkage groups (LG); names of markers are shown on the right. QTLs are depicted as vertical bars to the right of the LG. QTL detected for resistance to race 23 are indicated in black. QTLs identified for resistance to race 1545 are shown in gray. Co-location of resistance-associated genes encoding NB-LRR (NL) proteins with anthracnose QTLs are represented to the right of the QTLs.
See this image and copyright information in PMC

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