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. 2015 Feb 20:5:13.
doi: 10.1186/s13568-015-0095-6. eCollection 2015.

Microbial production of Propionic and Succinic acid from Sorbitol using Propionibacterium acidipropionici

Juliana C Duarte  1 , Gustavo P Valença  2 , Paulo J S Moran  3 , J Augusto R Rodrigues  3
Affiliations

Microbial production of Propionic and Succinic acid from Sorbitol using Propionibacterium acidipropionici

Juliana C Duarte et al. AMB Express. .

Abstract

Three sequential fermentative batches were carried out with cell recycle in four simultaneously operating bioreactors maintained at pH 6.5, 30°C, and 100 rpm. P. acidipropionici ATCC 4875 was able to produce propionic and succinic acid from sorbitol. The concentration of propionic acid decreased slightly from 39.5 ± 5.2 g L(-1) to 34.4 ± 1.9 g L(-1), and that of succinic acid increased significantly from 6.1 ± 2.1 g L(-1) to 14.8 ± 0.9 g L(-1) through the sequential batches. In addition, a small amount of acetic acid was produced that decreased from 3.3 ± 0.4 g L(-1) to 2.0 ± 0.3 g L(-1) through the batches. The major yield for propionic acid was 0.613 g g(-1) in the first batch and succinic acid it was 0.212 g g(-1) in the third batch. The minor yield of acetic acid was 0.029 g g(-1), in the second and third batches.

Keywords: Batch fermentation; Propionibacterium acidipropionici; Propionic acid; Sorbitol; Succinic acid.

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Figures

Figure 1
Figure 1
Profile of sorbitol fermentation by P. acidipropionici for the first batch operating in four independent vessels simultaneously.
Figure 2
Figure 2
Profile of sorbitol fermentation by P. acidipropionici for the second sequential batch operating in four independent vessels simultaneously.
Figure 3
Figure 3
Profile of sorbitol fermentation by P. acidipropionici for the third sequential batch operating in four independent vessels simultaneously.
Figure 4
Figure 4
Sorbitol and organic acids concentration at the end of each sequential batch (each batch was carried out in four independent vessels simultaneously). a) Sorbitol, b) Acetic acid, c) Succinic acid and d) Propionic acid.
See this image and copyright information in PMC

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