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. 2014 Jul 18;82(3):631-42.
doi: 10.3797/scipharm.1406-01. Print 2014 Jul-Sep.

Lack of Association between the Serotonin Transporter (5-HTT) and Serotonin Receptor (5-HT2A) Gene Polymorphisms with Smoking Behavior among Malaysian Malays

Nur Iwani A Rozak  1 , Imran Ahmad  2 , Siew Hua Gan  3 , Ruzilawati Abu Bakar  3
Affiliations

Lack of Association between the Serotonin Transporter (5-HTT) and Serotonin Receptor (5-HT2A) Gene Polymorphisms with Smoking Behavior among Malaysian Malays

Nur Iwani A Rozak et al. Sci Pharm. .

Abstract

An insertion/deletion polymorphism in the promoter region of the serotonin transporter gene (5-HTTLPR) and a polymorphism (rs6313) in the serotonin 2A receptor gene (5-HT2A) have previously been linked to smoking behavior. The objective of this study was to determine the possible association of the 5-HTTLPR and 5-HT2A gene polymorphisms with smoking behavior within a population of Malaysian male smokers (n=248) and non-smokers (n=248). The 5-HTTLPR genotypes were determined using the polymerase chain reaction (PCR) and were classified as short (S) alleles or long (L) alleles. The 5HT2A genotypes were determined using PCR-restriction fragment length polymorphisms (PCR-RFLP). No significant differences in the distribution frequencies of the alleles were found between the smokers and the non-smokers for the 5-HTTLPR polymorphism (x(2) = 0.72, P>0.05) or the 5HT2A polymorphism (x(2) = 0.73, P>0.05). This is the first study conducted on Malaysian Malay males regarding the association of 5-HTTLPR and 5HT2A polymorphisms and smoking behavior. However, the genes were not found to be associated with smoking behavior in our population.

Keywords: 5-HTTLPR; 5HT2A; Genetic polymorphism; Malaysian Malays male; PCR-RFLP.

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Figures

Fig. 1.
Fig. 1.
PCR products for amplification using 5-HTTLPR primers. Lane 1 and 4 show homozygous long allele (L), lane 2 and 5 show heterozygous short-long allele (S/L), while lane 3 and 6 show homozygous short allele (S)
Fig. 2.
Fig. 2.
a: PCR products for amplification using 5-HT2A primers, b: PCR-RFLP result after digestion with Hpa II restriction enzyme. Lane 1, 2, 5, 6, 7, 8 show mutant allele with 342 bp. Lane 9 shows wild type allele with 126 and 216 bp fragments and Lane 3 and 4 show wild type-mutant alleles
See this image and copyright information in PMC

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