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Review

Cardiomyocyte Impedance Assays

Sarah D. Lamore  1 , Clay W Scott  1 , Matthew F. Peters  1
Sarine Markossian  1 , Abigail Grossman  1 , Hannah Baskir  1 , Michelle Arkin  2 , Douglas Auld  3 , Christopher Austin  4 , Jonathan Baell  5 , Kyle Brimacombe  1 , Thomas D.Y. Chung  6 , Nathan P. Coussens  7 , Jayme L. Dahlin  8 , Viswanath Devanarayan  9 , Timothy L. Foley  10 , Marcie Glicksman  11 , Kirill Gorshkov  12 , Stefan Grotegut  13 , Matthew D. Hall  1 , Samuel Hoare  14 , James Inglese  1 , Philip W. Iversen  15 , Madhu Lal-Nag  16 , Zhuyin Li  12 , Jason R. Manro  13 , James McGee  17 , Allison Norvil  13 , Mackenzie Pearson  13 , Terry Riss  18 , Peter Saradjian  19 , G. Sitta Sittampalam  20 , Michael A. Tarselli  21 , O. Joseph Trask Jr.  22 , Jeffrey R. Weidner  23 , Mary Jo Wildey  24 , Kelli Wilson  1 , Menghang Xia  1 , Xin Xu  1
, editors.
In: Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004.
.
Affiliations
Review

Cardiomyocyte Impedance Assays

Sarah D. Lamore et al.

Excerpt

Cellular impedance assays have been broadly utilized as a label-free approach for toxicity and drug discovery screening. The xCELLigence RTCA Cardio instrument sets itself apart from other impedance technologies by its rapid data acquisition rate (12.9 msec). This speed is fast enough to detect the contractile activity of beating cardiomyocytes, offering a relatively high throughput and robust strategy to noninvasively monitor the effects of test compounds on cardiomyocyte function. This chapter introduces the fundamentals and applications of cardiomyocyte impedance assays. A protocol detailing cardiomyocyte culture, data acquisition, and data analysis using the xCELLigence RTCA Cardio system is provided and considerations for assay design and data interpretation are discussed.

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References

Literature Cited

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Additional References

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