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. 2015 May 14;13(18):5098-101.
doi: 10.1039/c5ob00462d.

Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide

Baolin Wu  1 , Na Wei, Vireak Thon, Mohui Wei, Zaikuan Yu, Yongmei Xu, Xi Chen, Jian Liu, Peng George Wang, Tiehai Li
Affiliations

Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide

Baolin Wu et al. Org Biomol Chem. .

Abstract

A biotinylated heparosan hexasaccharide was synthesized using a one-pot multi-enzyme strategy, in situ activation and transfer of N-trifluoroacetylglucosamine (GlcNTFA) to a heparin backbone significantly improved the synthetic efficiency. The biotinylated hexasaccharide could serve as a flexible core to diversify its conversion into heparan sulfate isoforms with potential biological applications and therapeutics.

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Figures

Figure 1
Figure 1
Fondaparinux (Arixtra®) and its analogues
Scheme 1
Scheme 1
Synthesis of GlcA-biotin conjugate 5
Scheme 2
Scheme 2
Sequential one-pot multi-enzyme synthesis of biotinylated heparosan hexasaccharide 10
See this image and copyright information in PMC

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