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. 2015:1301:213-24.
doi: 10.1007/978-1-4939-2599-5_17.

PFGE protocols to distinguish subspecies of Lactococcus lactis

Pascal Le Bourgeois  1 , Delphine Passerini, Michèle Coddeville, Maéva Guellerin, Marie-Line Daveran-Mingot, Paul Ritzenthaler
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PFGE protocols to distinguish subspecies of Lactococcus lactis

Pascal Le Bourgeois et al. Methods Mol Biol. 2015.

Abstract

Pulsed-field gel electrophoresis (PFGE), developed in the mid-1980s, rapidly became a "gold standard" method for analyzing bacterial chromosomes. Today, although outcompeted in resolution by alternative methods, such as optical mapping, and not applicable for high-throughput analyses, PFGE remains a valuable method for bacterial strain typing. Here, we describe optimized protocols for macrorestriction fingerprinting, characterization of plasmid complements, and gene localization by DNA-DNA hybridization of Lactococcus lactis genomes.

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