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Comment
. 2015 May;20(5):e10-1.
doi: 10.1634/theoncologist.2014-0280. Epub 2015 Apr 10.

Semiconductor-based sequencing of formalin-fixed, paraffin-embedded colorectal cancer samples

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Comment

Semiconductor-based sequencing of formalin-fixed, paraffin-embedded colorectal cancer samples

Albrecht Stenzinger et al. Oncologist. 2015 May.
No abstract available

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Figures

Figure 1.
Figure 1.
Representative results for colorectal cancer cases harboring either PIK3CA E545 variants or NRAS Q61R mutations. Data were obtained by semiconductor-based sequencing using the AmpliSeq cancer panel (CHPv2) and validated by conventional Sanger sequencing.

Comment in

  • In reply.
    Jia S, Zhang L, Latham GJ. Jia S, et al. Oncologist. 2015 May;20(5):e12. doi: 10.1634/theoncologist.2014-0357. Epub 2015 Apr 10. Oncologist. 2015. PMID: 25862746 Free PMC article. No abstract available.

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References

    1. Zhang L, Chen L, Sah S, et al. Profiling cancer gene mutations in clinical formalin-fixed, paraffin-embedded colorectal tumor specimens using targeted next-generation sequencing. The Oncologist. 2014;19:336–343. - PMC - PubMed
    1. Cancer Genome Atlas Network Comprehensive molecular characterization of human colon and rectal cancer. Nature. 2012;487:330–337. - PMC - PubMed
    1. Garraway LA, Lander ES. Lessons from the cancer genome. Cell. 2013;153:17–37. - PubMed
    1. Klauschen F, Andreeff M, Keilholz U, et al. The combinatorial complexity of cancer precision medicine. Oncoscience. 2014;1:504–509. - PMC - PubMed
    1. Endris V, Penzel R, Warth A, et al. Molecular diagnostic profiling of lung cancer specimens with a semiconductor-based massive parallel sequencing approach: feasibility, costs, and performance compared with conventional sequencing. J Mol Diagn. 2013;15:765–775. - PubMed

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