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. 2007 Mar;3(1):61-3.
doi: 10.1385/FSMP:3:1:61.

Real-time PCR

Victoria L Bowyer  1
Affiliations

Real-time PCR

Victoria L Bowyer. Forensic Sci Med Pathol. 2007 Mar.

Abstract

Research into the field of DNA has increased dramatically in the years since DNA profiling was first identified. Since then, techniques have been developed to identify individuals from DNA samples equivalent to a single cell. Development of these techniques meant that the ability to accurately quantitate the DNA in such samples was required to evolve at the same pace. As a result, there are a variety of quantitation protocols available, including the picogreen assay, slot blot systems, and real-time polymerase chain reaction (PCR). Real-time PCR is a relatively recent development but promises to be the most sensitive and accurate technique available.

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References

    1. J Forensic Sci. 2002 May;47(3):589-92 - PubMed
    1. J Forensic Sci. 2006 Jul;51(4):758-65 - PubMed
    1. J Forensic Sci. 2005 Jul;50(4):809-25 - PubMed
    1. J Forensic Sci. 2003 Nov;48(6):1280-7 - PubMed
    1. Nature. 1985 Jul 4-10;316(6023):76-9 - PubMed

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