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. 2015 Jun;19(6):283-7.
doi: 10.1089/gtmb.2015.0014. Epub 2015 Apr 15.

Downregulation of VANGL1 inhibits cellular invasion rather than cell motility in hepatocellular carcinoma cells without stimulation

Gokhan Ozan Cetin  1 , Asli Toylu  1 , Nese Atabey  1 , Zeynep Sercan  1 , Meral Sakizli  1
Affiliations

Downregulation of VANGL1 inhibits cellular invasion rather than cell motility in hepatocellular carcinoma cells without stimulation

Gokhan Ozan Cetin et al. Genet Test Mol Biomarkers. 2015 Jun.

Abstract

Aims: The Wnt planar cell polarity (PCP) pathway is one of the Wnt pathways which plays a critical role in cell proliferation and fate. The VANGL1 protein is one of Wnt-PCP pathway components. It is known that Wnt-PCP pathway has major roles in cell motility but its role in hepatocellular carcinoma (HCC) progression through invasion and metastasis needs to be clarified.

Methods: We silenced VANGL1 gene expression in the HepG2 HCC cell line by stable transfection with a vector containing siRNA template for VANGL1 and investigated the change in cell invasion and motility.

Results: Transfected cells with the siRNA template showed significantly suppressed invasive capacity when compared to controls although cellular motility was only slightly affected.

Conclusion: Our study showed a basal role for VANGL1 with respect to the invasive capacity of HCC cells. This suggests that the Wnt-PCP pathway may play a role in progression of HCC through cellular invasion but further studies are needed to clarify its role in cell motility.

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Figures

<b>FIG. 1.</b>
FIG. 1.
Relative quantification of VANGL1 expression with real-time polymerase chain reaction (PCR) in hepatocellular cancer cell lines.
<b>FIG. 2.</b>
FIG. 2.
(a) VANGL1 expression of HEP-G2 cells transfected with pSilencer plasmid. pSIL-VANGL1 cells carry the shRNA template for VANGL1 and pSIL where the control cells transfected with the control pSilencer vector. Relative quantification of VANGL1 expression with real-time PCR in transfected cells showing the degree of gene silencing (p=0.0009). *Indicates the significance. (b) Western blot analysis of VANGL1 expression in the cells transfected with siRNA.
<b>FIG. 3.</b>
FIG. 3.
The change in the number of (a) invasive (p=0.03) and (b) motile HEP-G2 cells after transfection with VANGL1 siRNA. *Indicates the significance. pSIL-VANGL1 cells carry the shRNA template for VANGL1 and pSIL where the control cells transfected with the control pSilencer vector.
See this image and copyright information in PMC

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