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. 1978 Apr;134(1):147-56.
doi: 10.1128/jb.134.1.147-156.1978.

Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli

J Lopilato, T Tsuchiya, T H Wilson

Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli

J Lopilato et al. J Bacteriol. 1978 Apr.

Abstract

Thiomethyl-beta-galactoside (TMG) accumulation via the melibiose transport system was studied in lactose transport-negative strains of Escherichia coli. TMG uptake by either intact cells or membrane vesicles was markedly stimulated by Na+ or Li+ between pH 5.5 and 8. The Km for uptake of TMG was approximately 0.2 mM at an external Na+ concentration of 5 mM (pH 7). The alpha-galactosides, melibiose, methyl-alpha-galactoside, and o-nitrophenyl-alpha-galactoside had a high affinity for this system whereas lactose, maltose and glucose had none. Evidence is presented for Li+-TMG or Na+-TMG cotransport.

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