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. 2015 Apr 17;23(1):24.
doi: 10.1186/s40199-015-0108-7.

The fruit extract of Berberis crataegina DC: exerts potent antioxidant activity and protects DNA integrity

Mohammad Charehsaz  1 , Hande Sipahi  2 , Engin Celep  3 , Aylin Üstündağ  4 , Özge Cemiloğlu Ülker  5 , Yalçın Duydu  6 , Ahmet Aydın  7 , Erdem Yesilada  8
Affiliations

The fruit extract of Berberis crataegina DC: exerts potent antioxidant activity and protects DNA integrity

Mohammad Charehsaz et al. Daru. .

Abstract

Background: Dried fruits of Berberis crataegina (Berberidaceae) have been frequently consumed as food garniture in Turkish cuisine, while its fruit paste has been used to increase stamina and in particular to prevent from cardiovascular dysfunctions in Northeastern Black Sea region of Turkey. This study investigated this folkloric information in order to explain the claimed healing effects as well as to evaluate possible risks.

Methods: Total phenolic, flavonoid and proanthocyanidin contents and antioxidant capacity of the methanolic fruit extract were evaluated through several in vitro assays. The cytotoxic and genotoxic effects of B. crataegina fruit extract were also assessed in both cervical cancer cell line (HeLa) and human peripheral blood lymphocytes.

Results: The extract showed protective effects against ferric-induced oxidative stress and had a relatively good antioxidant activity. It also ameliorated the H2O2 mediated DNA damage in lymphocytes, suggesting the protective effect against oxidative DNA damage.

Conclusion: The methanolic extract of B. crataegina fruits may be a potential antioxidant nutrient and also may exert a protective role against lipid peroxidation as well as oxidative DNA damage.

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Figures

Figure 1
Figure 1
The DNA integrity of HeLa cells treated with increasing concentrations of BCFE for 2 hours. *The tail % intensity was significantly increased when compared with the control (p < 0.05). Black bars representing the positive controls.
Figure 2
Figure 2
The DNA integrity of HeLa cells treated with increasing concentrations of BCFE for 24 hours. *The tail % intensity was significantly increased when compared with the control (p < 0.05). Black bars representing the positive controls.
Figure 3
Figure 3
Dose-dependent effects of BCFE on the DNA integrity of human peripheral blood lymphocytes. The H2O2 (50 and 100 μM) treated lymphocytes are the controls for the combined treatments (black bars). The white and black bars represent the mean tail % intensity values determined after the treatment period of 2 hours. *Significantly different from the related control. The viability of lymphocytes varied between 93.75% and 100% within the tested concentrations.
Figure 4
Figure 4
The cytotoxic effects of BCFE and SDS (positive control). The IC50 values of BCFE and SDS were 4.98 mg/mL and 0.055 mg/mL, respectively.
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