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. 2015 Feb 19:8:113.
doi: 10.1186/s13071-015-0708-6.

Nematicidal activity of Annona crassiflora leaf extract on Caenorhabditis elegans

Alan Rodrigues Teixeira Machado  1 , Sebastião Rodrigo Ferreira  2 , Felipe da Silva Medeiros  3 , Ricardo Toshio Fujiwara  4 , José Dias de Souza Filho  5 , Lúcia Pinheiro Santos Pimenta  6
Affiliations

Nematicidal activity of Annona crassiflora leaf extract on Caenorhabditis elegans

Alan Rodrigues Teixeira Machado et al. Parasit Vectors. .

Abstract

Background: The aim of this work was to investigate the potential nematicidal activity of Annona crassiflora leaf extract against Caenorhabditis elegans.

Methods: The hydroalcoholic leaf extract and its fractions (dichloromethane, ethyl acetate, methanol and water) were submitted to mobility assay against the roundworm Caenorhabditis elegans. GC-MS and NMR analysis were performed in order to identify metabolites.

Results: The dichloromethane and ethyl acetate fractions showed to be the most active among the hydroalcoholic leaf extracts and its four fractions. The percentages of C. elegans larvae immobility were 98.13 and 89.66%, respectively, at a concentration of 1000 μg.mL(-1). Besides some amino acids, palmitic acid methyl ester, 2-isopropyl-5-methylcyclohexanol, oleic acid methyl esther, stearic acid methyl ester, quercetin and kaempferol were also identified in these fractions.

Conclusion: The results indicated that of A. crassiflora leaf ethanolic extract has a good potential as a source for natural nematicide.

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Figures

Figure 1
Figure 1
Effect of oil isolated from the leaves of Annona crassiflora and 2-isopropyl-5-methylcyclohexanol on mobility of Caenorhabditis elegans.
Figure 2
Figure 2
C. elegans larvae treated with 2-isopropyl-5-methylcyclohexanol, after a period of 72 hours were (a) treated with Sytox (b) and treated with propidium iodide.
See this image and copyright information in PMC

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