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Case Reports
. 2015 Mar 18:11:66.
doi: 10.1186/s12917-015-0369-x.

Isolation of Leptospira interrogans serovar Hardjoprajitno from a calf with clinical leptospirosis in Chile

Miguel Salgado  1 , Barbara Otto  2 , Manuel Moroni  3 , Errol Sandoval  4 , German Reinhardt  5 , Sofia Boqvist  6 , Carolina Encina  7 , Claudia Muñoz-Zanzi  8
Affiliations
Case Reports

Isolation of Leptospira interrogans serovar Hardjoprajitno from a calf with clinical leptospirosis in Chile

Miguel Salgado et al. BMC Vet Res. .

Abstract

Background: Although Leptospira isolation has been reported in Chilean cattle, only serological evidence of serovar Hardjo bovis infection has been routinely reported. The present report provides characterization of the pathological presentation and etiology of a clinical case of leptospirosis in a calf from the Los Rios Region in Chile.

Case presentation: In a dairy herd in southern Chile, 11 of 130 calves died after presenting signs such as depression and red-tinged urine. One of these calves, a female of eight months, was necropsied, and all the pathological findings were consistent with Leptospira infection. A urine sample was submitted to conventional bacteriological analysis together with highly specific molecular biology typing tools, in order to unravel the specific Leptospira specie and serovar associated with this clinical case. A significant finding of this study was that the obtained isolate was confirmed by PCR as L. interrogans, its VNTR profile properly matching with L. interrogans Hardjoprajitno as well as its specific genomic identity revealed by secY gen.

Conclusion: Leptospira interrogans serovar Hardjoprajitno was associated with the investigated calf clinical case. This information adds to the value of serologic results commonly reported, which encourage vaccination improvements to match circulating strains. In addition, this finding represents the first case report of this serovar in Chilean cattle.

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Figures

Figure 1
Figure 1
Calf with clinical sign and pathological findings consistent with Leptospira infection.
Figure 2
Figure 2
PCR analysis of the polymorphism of nine representative VNTR loci. Amplification was performed on the VNTR 4, 7, 10, 23, 27, 29, 30, 31, 36 loci of L. interrogans strains.
Figure 3
Figure 3
ClustalW aligment for 202 bp fragment, SecY gen.
See this image and copyright information in PMC

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