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Case Reports
. 2015 May;21(5):833-6.
doi: 10.3201/eid2105.141992.

Comparative sequence analyses of La Crosse virus strain isolated from patient with fatal encephalitis, Tennessee, USA

Amy J Lambert, Rebecca Trout Fryxell, Kimberly Freyman, Armando Ulloa, Jason O Velez, Dave Paulsen, Robert S Lanciotti, Abelardo Moncayo
Case Reports

Comparative sequence analyses of La Crosse virus strain isolated from patient with fatal encephalitis, Tennessee, USA

Amy J Lambert et al. Emerg Infect Dis. 2015 May.

Abstract

We characterized a La Crosse virus (LACV) isolate from the brain of a child who died of encephalitis-associated complications in eastern Tennessee, USA, during summer 2012. We compared the isolate with LACV sequences from mosquitoes collected near the child's home just after his postmortem diagnosis. In addition, we conducted phylogenetic analyses of these and other sequences derived from LACV strains representing varied temporal, geographic, and ecologic origins. Consistent with historical findings, results of these analyses indicate that a limited range of LACV lineage I genotypes is associated with severe clinical outcomes.

Keywords: La Crosse virus; Tennessee; United States; children; encephalitis; mosquitoes; sequence; vector-borne infections; viruses.

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Figures

Figure
Figure
Phylogeny of medium segment sequences of selected La Crosse virus strains of varied temporal, geographic, and ecological origin. Taxon descriptions are restricted in some cases according to a limited amount of information in GenBank. A neighbor-joining method was used with 2,000 replicates for bootstrap testing. Scale bar represents 0.005 nt substitutions per site. Box indicates the area of phylogenetic interest.
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