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Comment
. 2015 May;35(10):1882-3.
doi: 10.1128/MCB.00020-15.

TPC1 Knockout Knocks Out TPC1

Robert Hooper  1 , Dev Churamani  1 , Sean M Davidson  2 , Yaping Lin-Moshier  3 , Timothy F Walseth  3 , Sandip Patel  1 , Jonathan S Marchant  4
Affiliations
Comment

TPC1 Knockout Knocks Out TPC1

Robert Hooper et al. Mol Cell Biol. 2015 May.
No abstract available

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Figures

FIG 1
FIG 1
TPC1 protein is absent in transgenic TPC1 mice. (A) Photolabeling data using 5N3-[32P]NAADP showing specific photolabeling of an ∼23-kDa candidate NAADP binding protein in mouse pancreatic samples from a matched littermate mouse (TPC1+/+ [left]) and a TPC1 knockout mouse (TPC−/− [right]; Tpcn1LEXKO-471). Further detail is available in reference . (B) Schematic of TPC1 membrane topology to highlight the COOH-terminal epitope (red) present in wild-type TPC1 and the putative NH2-terminal truncation of TPC1B (the missing sequence is shown with a black line). (C and D) Western blot analysis of liver samples from wild-type (+/+) and Tpcn1LEXKO-471 (–/–) mice described in reference using an antibody raised to the C terminus of TPC1 (ab80961; Abcam). (D) Samples were treated with peptide-N-glycosidase F (PNGase F) (right) to remove N-linked oligosaccharides as described in reference . Note the absence of major immunoreactive bands (solid arrowheads) in the transgenic mice. Bands present in both samples (open arrowheads) are likely nonspecific, but we cannot rule out the possibility of severely truncated, nonfunctional TPC1 variants that escape inactivation. Numbers at the left of the gels are molecular masses (in kilodaltons).
See this image and copyright information in PMC

Comment in

  • Reply to "TPC1 Knockout Knocks Out TPC1".
    Ruas M, Galione A, Parrington J. Ruas M, et al. Mol Cell Biol. 2015 May;35(10):1884. doi: 10.1128/MCB.00083-15. Mol Cell Biol. 2015. PMID: 25900975 Free PMC article. No abstract available.

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