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. 2015 Apr 24;10(4):e0125069.
doi: 10.1371/journal.pone.0125069. eCollection 2015.

Increased plasma clot permeability and susceptibility to lysis are associated with heavy menstrual bleeding of unknown cause: a case-control study

Piotr Szczepaniak  1 , Michał Zabczyk  2 , Anetta Undas  3
Affiliations

Increased plasma clot permeability and susceptibility to lysis are associated with heavy menstrual bleeding of unknown cause: a case-control study

Piotr Szczepaniak et al. PLoS One. .

Abstract

Background: Formation of compact and poorly lysable clots has been reported in thromboembolic disorders. Little is known about clot properties in bleeding disorders.

Objectives: We hypothesized that more permeable and lysis-sensitive fibrin clots can be detected in women with heavy menstrual bleeding (HMB).

Methods: We studied 52 women with HMB of unknown cause and 52 age-matched control women. Plasma clot permeability (Ks), turbidity and efficiency of fibrinolysis, together with coagulation factors, fibrinolysis proteins, and platelet aggregation were measured.

Results: Women with HMB formed looser plasma fibrin clots (+16% [95%CI 7-18%] Ks) that displayed lower maximum absorbancy (-7% [95%CI -9 - -1%] ΔAbsmax), and shorter clot lysis time (-17% [95%CI -23 - -11%] CLT). The HMB patients and controls did not differ with regard to coagulation factors, fibrinogen, von Willebrand antigen, thrombin generation markers and the proportion of subjects with defective platelet aggregation. The patients had lower platelet count (-12% [95%CI -19 - -2%]), tissue plasminogen activator antigen (-39% [95%CI -41 - -29%] tPA:Ag), and plasminogen activator inhibitor-1 antigen (-28% [95%CI -38 - -18%] PAI-1:Ag) compared with the controls. Multiple regression analysis upon adjustment for age, body mass index, glucose, and fibrinogen showed that decreased tPA:Ag and shortened CLT were the independent predictors of HMB.

Conclusions: Increased clot permeability and susceptibility to fibrinolysis are associated with HMB, suggesting that altered plasma fibrin clot properties might contribute to bleeding disorders of unknown origin.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. ROC curve for tissue plasminogen activator antigen (tPA:Ag).
AUC denotes area under curve; SE, standard error; CI, confidence intervals.
Fig 2
Fig 2. ROC curve for clot lysis time (CLT).
AUC denotes area under curve; SE, standard error; CI, confidence intervals.
Fig 3
Fig 3. Representative SEM images of plasma fibrin clots.
(A) a patient with heavy menstrual bleeding (HMB). (B) a healthy control subject. Magnification 5000x. Scale bar 5 μm.
See this image and copyright information in PMC

References

    1. Marret H, Fauconnier A, Chabbert-Buffet N, Cravello L, Golfier F, Gondry J, et al. (2010) Clinical practice guidelines on menorrhagia: management of abnormal uterine bleeding before menopause. Eur J Obstet Gynecol Reprod Biol 152: 133–137. 10.1016/j.ejogrb.2010.07.016 - DOI - PubMed
    1. Vessey MP, Villard-Mackintosh L, McPherson K, Coulter A, Yeates D. (1992) The epidemiology of hysterectomy: findings in a large cohort study. Br J Obstet Gynaecol 99: 402–407. - PubMed
    1. Cote I, Jacobs P, Cumming D. (2002) Work loss associated with increased menstrual loss in the United States. Obstet Gynecol 100: 683–687. - PubMed
    1. Kujovich JL. (2005) von Willebrand’s disease and menorrhagia: prevalence, diagnosis, and management. Am J Hematol 79: 220–228. - PubMed
    1. Philipp CS, Dilley A, Miller CH, Evatt B, Baranwal A, Schwartz R, et al. (2003) Platelet functional defects in women with unexplained menorrhagia. J Thromb Haemost 1: 477–484. - PubMed

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