Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa

Abstract

The study of mitochondrial composition, biogenesis, and metabolism requires the isolation of intact functional mitochondria, often in relatively large quantities sufficient for downstream biochemical and proteomic analysis. Here we describe techniques optimized for the isolation of functional mitochondria from a variety of tissues from the model monocot and dicot species Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa).