Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Jun;12(6):519-22.
doi: 10.1038/nmeth.3370. Epub 2015 Apr 27.

G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

Iain C Macaulay  1 , Wilfried Haerty  2 , Parveen Kumar  3 , Yang I Li  2 , Tim Xiaoming Hu  2 , Mabel J Teng  4 , Mubeen Goolam  5 , Nathalie Saurat  6 , Paul Coupland  7 , Lesley M Shirley  7 , Miriam Smith  7 , Niels Van der Aa  3 , Ruby Banerjee  8 , Peter D Ellis  7 , Michael A Quail  7 , Harold P Swerdlow  7 , Magdalena Zernicka-Goetz  5 , Frederick J Livesey  6 , Chris P Ponting  9 , Thierry Voet  10
Affiliations

G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

Iain C Macaulay et al. Nat Methods. 2015 Jun.

Abstract

The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

PubMed Disclaimer

References

    1. Nat Biotechnol. 2002 Apr;20(4):387-92 - PubMed
    1. Nature. 2009 Apr 9;458(7239):719-24 - PubMed
    1. Genome Res. 2002 Apr;12(4):656-64 - PubMed
    1. Cell Rep. 2012 Sep 27;2(3):666-73 - PubMed
    1. Front Oncol. 2013 Dec 31;3:320 - PubMed

Publication types