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. 2014 Dec 24:14:335.
doi: 10.1186/s12866-014-0335-y.

Microbiological diagnostic procedures for respiratory cystic fibrosis samples in Spain: towards standard of care practices

Juan de Dios Caballero  1   2 , Rosa del Campo  3   4 , Marta Tato  5   6 , Elia Gómez G de la Pedrosa  7   8 , Marta Cobo  9 , Carla López-Causapé  10   11 , Enrique Gómez-Mampaso  12 , Antonio Oliver  13   14 , Rafael Cantón  15   16 ; Spanish Network for Cystic Fibrosis Microbiology Laboratories
Collaborators, Affiliations

Microbiological diagnostic procedures for respiratory cystic fibrosis samples in Spain: towards standard of care practices

Juan de Dios Caballero et al. BMC Microbiol. .

Abstract

Background: The microbiological procedures for cystic fibrosis (CF) samples of 17 participating Spanish centers were examined to verify their compliance with current international and national guidelines and to implement the best standards of care for microbiology practices. A 47-item questionnaire covering different CF microbiology aspects was sent to participant laboratories. Telephone interviews were performed when necessary. Data about samples processing for bacteria, mycobacteria and fungi were collected.

Results: Gene sequencing (71%), MALDI-TOF (59%) or both (94%) were available for most laboratories. Susceptibility testing was performed by automated microdilution systems (94%) and manual diffusion methods (59%). However, a low use of selective media for Staphylococcus aureus (59%) and Burkholderia cepacia complex (71%), and of epidemiological typing methods (41%) was reported.

Conclusions: Most Spanish laboratories are in agreement with consensus guidelines for the processing of CF respiratory samples, but need to improve in the use of specific selective media and typing methods for epidemiologic studies.

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Figures

Figure 1
Figure 1
CF samples processed weekly by participant laboratories.
See this image and copyright information in PMC

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