Electrical properties of cultured renal tubular cells (OK) grown in confluent monolayers

Abstract

OK cells grown to confluent monolayers were investigated by microelectrode techniques and microinjection. Cell membrane potential difference (PDm) in bicarbonate-free solution is -61.8 +/- 0.6 mV (n = 208), cell membrane resistance (Rm) amounts to 1.4 +/- 0.2 k omega. cm2 (n = 8). The apparent transference number for potassium (t'k+) is 71 +/- 3% (n = 28) and can be reduced by 3 mmol/l BaCl2 to 7.5 +/- 4.0%; (n = 8). In the presence of extracellular CO2 and HCO3- (pH 7.4) the cells acidify by 0.34 +/- 0.05 pH units (n = 12). This leads to a depolarization of PDm by 8.4 +/- 1.8 mV (n = 8), an increase in Rm by 49 +/- 10% (n = 10), and a reduction of K+-conductance to 63 +/- 5% (n = 13). Intracellular acidification by the NH4Cl-prepulse technique also inhibits K+-conductance and depolarizes the membrane. Recovery from an intracellular acid load is reflected by cell membrane repolarization. This recovery can be inhibited by amiloride (10(-3) mol/l). Na+- and Cl- -conductances could not be detected. The transepithelial resistance (Rte) of OK cell monolayers 1 day after plating is 41 +/- 6 omega.cm2 and decreases with time after plating. Intercellular communication (electrical or dye coupling) was not observed.

Conclusions: 1. The membrane potential of OK cells is largely determined by a pH-sensitive, barium-blockable K+-conductance. 2. Amiloride-blockable Na+/H+-exchange is reflected by membrane potential changes via this K+-conductance. 3. Monolayers of OK cells are electrically leaky.