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. 2015:2015:416479.
doi: 10.1155/2015/416479. Epub 2015 Apr 5.

Proposal of a Screening MIRU-VNTR Panel for the Preliminary Genotyping of Mycobacterium bovis in Mexico

Enrique Bolado-Martínez  1 , Iliana Benavides-Dávila  1 , Maria Del Carmen Candia-Plata  2 , Moisés Navarro-Navarro  1 , Magali Avilés-Acosta  3 , Gerardo Álvarez-Hernández  2
Affiliations

Proposal of a Screening MIRU-VNTR Panel for the Preliminary Genotyping of Mycobacterium bovis in Mexico

Enrique Bolado-Martínez et al. Biomed Res Int. 2015.

Abstract

Mycobacterium bovis is the major causative agent of bovine tuberculosis, one of the most relevant zoonoses in the world, and affects a wide range of wild and domesticated animals. Development of screening panels in mycobacterial genotyping, according to specific geographical regions, is strongly needed. The aim of this study is to select a panel, constituted by highly polymorphic MIRU-VNTR loci, to discriminate clinical isolates of M. bovis in Mexico. In this study, 65 isolates of M. bovis obtained from clinical bovine samples proceeding from different geographic regions of Mexico were identified by phenotypic and genotypic tests and subsequently genotyped by a 24-locus MIRU-VNTR panel. The most polymorphic loci were selected to build a panel with a high discriminatory power similar to the 24-locus panel results. A panel of seven elements (QUB 11a, MIRU 26, ETR-A, QUB 26, MIRU 16, MIRU 27, and MIRU 39) with the highest allelic diversity showed an appropriate differentiation. The selected MIRU-VNTR elements, according to the regional allelic variability, may be used in the preliminary genotyping of Mycobacterium bovis isolates in Mexico.

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Figures

Figure 1
Figure 1
Map of Mexico and geographical origin of the 65 Mycobacterium bovis isolates included in this study.
Figure 2
Figure 2
Dendrogram generated by using categorical character and the UPGMA clustering method with 24-locus MIRU-VNTR panel results of 65 isolates of M. bovis and M. tuberculosis strain H37Rv.
Figure 3
Figure 3
Dendrogram generated by using categorical character and the UPGMA clustering method with 7-locus MIRU-VNTR panel results of 65 isolates of M. bovis and M. tuberculosis strain H37Rv.
See this image and copyright information in PMC

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