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. 1989 Dec;86(23):9119-23.
doi: 10.1073/pnas.86.23.9119.

Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene

A Laban  1 , D F Wirth
Affiliations

Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene

A Laban et al. Proc Natl Acad Sci U S A. 1989 Dec.

Abstract

We report a transient expression transfection system in Leishmania enriettii. A hybrid gene containing an intergenic region of the alpha-tubulin cluster and the bacterial chloramphenicol acetyltransferase (CAT; EC 2.3.1.28) gene is expressed after transfection of L. enriettii with the hybrid plasmid. The expression of the CAT gene is dependent on the presence of sequences from the alpha-tubulin gene. The hybrid gene is also active in Leishmania braziliensis and Leishmania major.

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References

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