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. 1989 Dec;86(23):9149-53.
doi: 10.1073/pnas.86.23.9149.

Thymine dimer formation as a probe of the path of DNA in and between nucleosomes in intact chromatin

J R Pehrson  1
Affiliations

Thymine dimer formation as a probe of the path of DNA in and between nucleosomes in intact chromatin

J R Pehrson. Proc Natl Acad Sci U S A. 1989 Dec.

Abstract

Photo-induced thymine dimer formation was used to probe nucleosome structure in nuclei. The distribution of thymine dimers in the nucleosome and recent studies of the structure of thymine dimer-containing DNA suggest that the rate of thymine dimer formation is affected by the direction and degree of DNA bending. This premise was used to construct a model of the path of DNA in the nucleosome, which has the following features. (i) There are four regions of sharp bending, two which have been seen previously by x-ray crystallography of the core particle. (ii) The DNA in H1-containing nucleosomes deviates from its superhelical path near the midpoint; this is not seen with H1-stripped chromatin. (iii) The internucleosomal (linker) DNA appears to be relatively straight.

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